• 平行板流动小室
  • 系统完整结构图



    NE-1000程控注射泵(现货

    平 行 板 流 动 小 室

  • (Parallel-Plate Flow Chamber)
  • 描 述:
  • 平行板流动小室(Parallel-Plate Flow Chamber)是体外研究细胞力学最常用的模型之一,用来模拟体内各种细胞处于生理环境中,暴露在动态液体流作用下所受到的流体剪应力。体外细胞的代谢反应与所受到的壁面剪应力密切相关。
  • 典型的平行板流动小室由三个部分组成:聚碳酸酯分液室,硅胶垫圈和玻璃盖玻片。分液室构成流动小室的一侧,包括进液口,出液口和真空槽。垫圈的厚度决定流程长度。盖玻片构成流动小室的另一侧,其上覆盖细胞外基质蛋白(ECM),血管细胞或其他待研究的生物材料。真空形成一个密封环境将三个部件紧密连接在一起,同时也确保通道高度的一致性。
  • 优 势:
  • 1.平行板流动小室能够产生生理学范畴内的壁面剪应力:0.01-30 dyn/cm2。通过注射泵以可控的运动力将液体(如抗凝的全血或者细胞悬液)注入小室并流进固定化基质的方法产生剪应力。
  • 2.装置设计,组装和操作简单,方便;
  • 3.可以在设定的时间-周期内研究稳定剪应力对细胞的影响;也可以随实验需求调节流速和剪应力;
  • 细胞可在流动条件下生长,能够在显微镜下随时观察生长状态;或者利用视频显微镜实时监控细胞状态;
  • 应 用:
  • 1.模拟体内血液流动产生的血流剪切力,研究不同剪应力下白细胞和内皮细胞之间的动态粘附作用或白细胞受体-配体间的相互作用等;
  • 2.利用平行板流动小室产生剪应力模拟肿瘤细胞的体内生长环境,从而研究肿瘤细胞的增殖,吸附和转移机制。
  • 3.细胞趋化实验中作为药物测试体系,基于白细胞-内皮细胞粘附过程作为新药物的运输体系;
  • 产品介绍:
  • Glycotech公司提供两种平行板流动小室:Rectangular Flow Chamber(矩形流动小室)和Circular Flow Chamber(圆形流动小室),含有平行流动小室装置需要的基本组成部件。客户根据自身实验需求选择合适的产品。
  • (1).Rectangular Flow Chamber(用于标准显微载玻片)
  • Rectangular Flow Chamber Kit(Cat#:31-010)
  • 组成成分:
  • 1个 流动板 (灌注的聚丙烯酸材质)
  • 4个 硅橡胶垫圈(每种尺寸各2个)
  • Flow Width = 1.00 cm, Thickness = 0.005 in
  • Flow Width = 1.00 cm, Thickness = 0.010 in
  • 24个 聚丙烯管接头(每种尺寸各6个)
  • Threaded 1/16 in. straight and L-connectors
  • Male and Female taper luer to 1/16 in. connectors
  • 硅橡胶实验软管(1/16 in. ID x 10 ft.)
  • (2).Circular Flow Chamber(用于35mm组织培养皿)
  • Circular Flow Chamber Kit(Cat#:31-001)
  • 组成成分:
  • 2个 流动板 (灌注的聚丙烯酸材质,带有不同的进出口设计)
  • 8个硅橡胶垫圈(每种尺寸各2个)
  • Flow Width = 0.25 cm, Thickness = 0.005 in
  • Flow Width = 0.25 cm, Thickness = 0.010 in
  • Flow Width = 0.50 cm, Thickness = 0.010 in
  • Flow Width = 1.00 cm, Thickness = 0.010 in
  • 24个聚丙烯管接头(每种尺寸各6个)
  • Threaded 1/16 in. straight and L-connectors
  • Male and Female taper luer to 1/16 in. connectors
  • 硅橡胶实验软管(1/16 in. ID x 10 ft.)
  • 产品订购:
  • 图片
    品牌
    名称
    货号
    规格
    价格
    31-010
    Glycotech
    Rectangular Flow Chamber Kit
    31-010
    1 kit
    询价
    31-001
    Glycotech
    Circular Flow Chamber Kit
    31-001
    1 kit
    询价
  • 若对产品感兴趣,欢迎来电咨询。
  • 世联博研北京技有限公司 免费电话:400-650-8506 E-mail:slby800@163.com
  • 应用文献:
  • 1.Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850
  • 2. FLOW CHAMBER FOR STUDYING CELL ATTACHMENT TO OPAQUE SUBSTRATES. EDUARDO ALBERTO REYES.
  • 3. Physiologic Stress-Mediated Signaling in the Endothelium. Cynthia A.
  • 4. Covalent Immobilization of P-Selectin Enhances Cell Rolling. Seungpyo Hong.
  • 5. Improvements to parallel plate flow chambers to reduce reagent and cellular requirements. David C Brown.
  • 6. Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium.Georg Wiese.
  • 7. Dynamic Flow Assay in a Parallel Plate Flow Chamber

  • John T. Patton~GlycoTech Corporation, Rockville, Maryland 20850
  • Flow assays allow visualization of cell adhesion under well-defined wall shear stress. The visualization of the different events of cell adhesion can be quantified by selective image acquisition and subsequent image processing. Flow assays are uniquely suited to the investigation of adhesive events which occur very rapidly in a time scale shorter than that of most static adhesion assays. In addition, events subsequent to the initial events can be studied such as cell stabilization and spreading giving some insight into the kinetics of particular cell-cell or cell-substrate adhesive behavior.
  • Materials:

    Microscopy:
  • Inverted-stage microscope configured for phase contrast and fluorescence operation
  • Objective lens (6.3 x, 10 x, 40 x)
  • Stage incubator
  • Biological:
  • Cell suspension (neutrophils, cancer cells)
  • Cell monolayer or coated substrate in 35 mm dishes
  • Adhesion media (culture media serum-free with 12 mM Hepes)
  • Fibronectin (human plasma)
  • Bovine serum albumin (BSA)
  • Flow Chamber System:
  • Flow chamber deck with gasket and tube fittings
  • 35 mm tissue culture dishes
  • Harvard syringe pump
  • Glass syringes to fit on pump (3, 5, 20, or 60 cc)
  • Silastic tubing
  • Stopcocks
  • Vacuum pump
  • Computer/Electronics:
  • Digital image acquisition and processing system
  • Display monitor
  • Video recorder
  • CCD camera with controller
  • Time-date generator
  • Computer with interface to image system
  • Storage device for images
  • Protocol:

  • Cell Monolayer Preparation
  • 1. Coat dishes with human fibronectin (FN) by adding 1 ml of 5.5?g/ml FN solution to each dish.
  • 2. Incubate for 30 min. at room temperature.
  • 3. Aspirate solution out of each dish.
  • 4. Add 2 ml of cell suspension (Huvecs, CHO cells) to each dish at seeding density of 0.5-3.0 x 105 cells/ml depending on time required to reach confluence.
  • 5. After 1-5 days, use dishes with confluent monolayer in flow assay. Feed cells every 2-3 days, but usually not with 48 hrs. of flow assay.
  • Coated Substrate Preparation
  • 1. Outline a coating region (5 mm diameter) for coating substrate in the center of each dish with marking pen.
  • 2. Add 20?l of solution containing substrate at a concentration of 10?g/ml to coating region. Incubate 1 hr.
  • 3. Aspirate off liquid, add 20?l of 1% BSA to block for 1 hr.
  • 4. Aspirate off BSA, add 20?l of inhibitor for 1 hr.
  • 5. Dishes are ready for use in flow assay.
  • Flow Assay Using Parallel Plate Flow Chamber
  • 1. Turn on stage incubator and warm adhesion media to 37?C 1 hr. prior to beginning flow assay.
  • 2. Assemble flow system apparatus connecting inlet, outlet, and vacuum lines to the flow chamber deck. Fill system with media and remove all air from system.
  • 3. Fill inlet reservoir with cell suspension. For assays using cell monolayers, 106 cells/ml is recommended. For coated substrate assays, use 105 cells/ml.
  • 4. Attach dish to flow chamber deck by holding the deck inverted, place a small bubble of media on flow path area, then place 35 mm dish on the deck. Vacuum will hold dish on deck. Make sure dish was attached with no air bubbles in the flow path.
  • 5. Place assembled chamber on microscope stage.
  • 6. Initiate flow of cells syringe pump connected to outlet flow chamber at a shear stress in the range of 0.1-4.0 dynes/cm2.
  • 7. Allow cells to flow for sufficient time to get an adequate number of cells interacting with the cell monolayer or coated surface. Generally 3-10 min. is used.
  • 8. Begin image acquisition. Collect images at 7-10 locations on the dish. Generally 3 dishes at a given experimental condition gives enough data to show statistical differences between treatments.
  • 9. After images are acquired on all dishes, perform image analysis to quantify the flow assay.
  • References:
  • (1) Lawrence, M.B., McIntire, L.V., Eskin, S.K., (1987), Effect of flow on polymorphonuclear leukocyte/ endothelial cell adhesion, Blood 70: 1284-1290.
  • (2) Patton, J.T., Menter, D.G., Benson, D.M., Nicolson, G.L., McIntire, L.V., (1993), Computerized analysis of tumor cells flowing in a parallel plate chamber to determine their adhesion stabilization lag time, Cell Motility and the Cytoskeleton 26: 88-98.
  • (3) Jones, D.A., Abbassi, O., McIntire, L.V., McEver, R.P., Smith, C.W., (1993), P-selectin mediates neutrophil rolling on histamine-stimulated endothelial cells, Biophysical Journal 65: 1560-1569.

    NE-1000程控注射泵,货促销

    NE-1000 Programmable Single Syringe Pump

    syringepump精密可编程注射泵NE-1000现货促销

    技术参数:

    注射器的容量达到60ml
    注射速率可以从0.73uL/hr-2100mL/hr调节
    节省空间的设计,小巧结实的外观,为你实验室节省空间



    主要特点:

    1.有注入和回抽功能
    2.可编程控制,最大41阶命令(注射的速率、注射的容量、插入暂停)
    3.一台电脑可以控制100台注射泵
    4.注射的精度小于正负1%

    New Era系列注射器微量注射泵可用于动物实验,同时具有注射和回抽功能,可控制流速,可通过程序控制注射流量。相比较其它同类产品,New Era泵提供了更强的功能,然而价格确比其它品牌要低。这让New Era泵嬴得了大量的客户。客户向其它的同事推荐这种微量注射泵。对于需要双泵的用户而言,New Era系统的优势是巨大的。它以不高于其它品牌双通道泵的价格,提供两台单通道泵。这两台泵可以单独控制,从事不同的实验,也可以互联,运行相同的参数或程序。功能和实用性均远超其它厂家的双通道泵。

    New Era微量注射泵可兼容市场上绝大部分的注射器。

    主要特性:

    1. 可单机操作也可电脑控制 (RS-232插头)

    1. 可注射也可回抽 (双向)

    1. 可用1~60cc注射器

    1. 可选择注射流量及流速控制程序,有41种选择

    1. 可将多个泵连接组合成一个系统 (双筒), 最大可达100泵;

    1. 单筒NE-1000、双筒NE-4000, 6筒NE-1600、8筒NE-1800整机选择.

    1. 注射速率pumping rate:

    NE-1000: 0.73 ml/小时~2100ml/小时

    NE-4000: 0.73 ml/小时~2100ml/小时

    NE-1600: 0.568 ml/小时~1337ml/小时

    NE-1800: 0.568 ml/小时~380 ml/小时

    技术参数:

    注射器尺寸    1-60ml,or 0.5-5μl微量注射器

    注射器数目     1,6,8 依据泵的型号变化

    驱动方式     步进马达,1/8 to 1/2步进方式

    转轴最大步进数  400/s

    马达步进距离   0.850μm(1/2step)

    马达/转轴齿数    15/28

    速度(max/min) 5.1cm/min 0.0042cm/hr

    泵流量       最大:1699mL/hr,60ml注射器

              最小:0.73μL/hr,1ml注射器

    最大推力      35lb 最小流速时

              18lb 最大流速时

    程序数       41

    电源        220V,50-60Hz

    尺寸       22.9×14.6×11.4 cm

    重量       1.6kg