PTEN (pS380/T382/T383) Antibody is a Rabbit Polyclonal antibody against PTEN (pS380/T382/T383). Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.
| Target |
PTEN (pS380/T382/T383) |
| Reactivity |
Human, Mouse, Rat |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Tested Applications |
WB, IHC, IF/ICC |
| Recommended dilutions |
WB: 1/500 - 1/1000, IHC: 1/50 - 1/100, IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Synthetic Peptide. A phospho specific peptide corresponding to residues surrounding S380/T382/T383 of human PTEN. |
| Purification |
Affinity purified. |
| Form |
Liquid |
| Isotype |
IgG |
| Conjugation |
Unconjugated |
| Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Molecular Weight |
Calculated MW: 19 kDa/47 kDa/64 kDa
Observed MW: 55 kDa |
| Swiss Prot |
P60484
|
| GeneID |
5728
|
| Gene Symbol |
PTEN |
| Enzyme Commission Number |
EC 3.1.3.16, EC 3.1.3.67, EC 3.1.3.48 |
| Concentration |
> 1 mg/ml |
| Buffer |
PBS, pH 7.3, 0.02% sodium azide, 50% glycerol. |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |