H2AFX Antibody is a Rabbit Polyclonal antibody against H2AFX. Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability.

Target	H2AFX
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Tested Applications	WB, IHC
Recommended dilutions	WB: 1/500 - 1/1000, IHC: 1/50 - 1/100. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Recombinant protein of human H2AFX.
Purification	Affinity purified.
Form	Liquid
Isotype	IgG
Conjugation	Unconjugated
Storage	Aliquot and store at 2°C to 8°C upon receipt.
Molecular Weight	Calculated MW: 15 kDa Observed MW: 15 kDa
Swiss Prot	P16104
GeneID	3014
Gene Symbol	H2AFX
Concentration	> 1 mg/ml
Buffer	PBS, pH 7.3, 0.02% sodium azide.
UNSPSC Code	12352203
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.