| 产品详情 |
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| Antigenic Specificity | ATG5 |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human, mouse, rat, porcine, bovine, primate, Xenopus, zebrafish |
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| Isotype | n/a |
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| Format | immunogen affinity purified |
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| Size | 0.1 ml |
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| Concentration | n/a |
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| Applications | Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Radioimmunoassay, Proximity Ligation Assay, Knockout Validated. In Western Blot, a band is seen 56 kDa representing the ATG5-ATG12 complex, the molecular weight of human ATG5 is 33 kDa. In ICC/IF, cytoplasmic staining was observed in SY5Y cells. In IHC-P, staining was observed in the cytoplasm of human hepatocytes and mouse intestine tissues. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunoprecipitation reported in scientific literature (PMID 24705551). Use in radioimmunoassay reported in scientific literature (PMID: 28651493). PLA data from independent lab. Use in electron microscopy reported in scientific literature (PMID 27219062). Use in ELISA reported in scientific literature (PMID: 25484078). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The ATG5 Antibody from Novus Biologicals is a rabbit polyclonal antibody to ATG5. This antibody reacts with human, mouse, rat, porcine, bovine, primate, xenopus, zebrafish. The ATG5 Antibody has been validated for the following applications: Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Radioimmunoassay, Proximity Ligation Assay, Knockout Validated. This is selective for the full-length and calpain cleaved isoform proteins |
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| Immunogen | A synthetic peptide made to an N-terminal region of the human ATG5 protein (within residues 1-50) [Swiss-Prot Q9H1Y0] |
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| Other Names | APG5 autophagy 5-like (S. cerevisiae), APG5LS. cerevisiae)-like, ASPAPG5-LIKE, ATG5 autophagy related 5 homolog (S. cerevisiae), Autophagy protein 5 |
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| Gene, Accession # | ATG5, Gene ID: 9474, SwissProt: Q9H1Y0 |
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| Catalog # | NB110-53818 |
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| Price | |
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| Order / More Info | ATG5 Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | PubMed: 18073215, 18196969, 18849966, 19305394, 19571141, 19654319, 19898471, 21062285, 21075354, 21228817, 21342660, 21353614, 21363932, 21555592, 21572516, 21690574, 21946213, 21954231, 22127234, 22150682, 22223827, 22395361, 22405854, 22440612, 22509024, 22730392, 22732841, 22840404, 22875977, 22929803, 22934255, 23039021, 23195496, 23242206, 23341467, 23348250, 23359526, 23364696, 23427266, 23434181, 23446737, 23519075, 23658518, 23681233, 23691217, 23733886, 23740229, 23768496, 23800883, 23884556, 23894198, 23894332, 23907538, 23918802, 23965851, 24012002, 24086672, 24100123, 24160177, 24198074, 24240988, 24262968, 24277826, 24317199, 24376535, 24441423, 24443716, 24458007, 24466199, 24528867, 24705551, 24813611, 24875860, 24895572, 24928681, 25046114, 25142602, 25293350, 25323583, 25341032, 25355898, 25375375, 25484078, 25485521, 25498006, 25585051, 25622137, 25643395, 25650339, 25669150, 25708836, 25753040, 25795167, 25804753, 25825498, 25827954, 25879848, 25946189, 26022126, 26075877, 26103419, 26120775, 26183773, 26212128, 26217309, 26237736, 26255724, 26306675, 26412641, 26487561, 26492575, 26698918, 26764007, 26814135, 27025389, 27067261, 27172265, 27184837, 27214279, 27219062, 27346059, 27509858, 27613096, 27655644, 27775547, 27810915, 28122966, 28132808, 28231469, 28428343, 28591635, 28600173, 28651493, 28779098, 28801209, 28953980, 29042667, 29107505, 29166823, 29174892, 29207660, 29222450, 29231257, 29361549, 29654322, 29685904, 29692779, 29940785, 30210920, 30296435, 30297526, 30340022, 30629163, 30766882, 30767704 |
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