| 产品详情 |
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| Product Name | nanA, 1-297aa, E.coli, His tag, E.coli |
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| Description | Purity: > 95% by SDS - PAGE Format: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol. NanA, also known as N-acetylneuraminate lyase, belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. NanA catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetyl-D-mannosamine. This protein was inhibited by reduction with NaBH4 in the presence of the substrate, indicating that it belongs to the Schiff-base-forming Class I aldolases. NanA was strongly inhibited by Cu2+ ions, p-chloromercuribenzoate and N-bromosuccinimide, and also inhibited competitively by the reaction product, pyruvate, and its structurally related compounds, dihydroxyacetone and DL-glyceraldehyde. Recombinant E.coli nanA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
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| Size | 0.5mg, 100ug, 20ug |
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| Concentration | n/a |
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| Applications | SDS-PAGE |
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| Other Names | N-acetylneuraminate lyase, npl |
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| Gene, Accession, CAS # | NP 417692 |
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| Catalog # | ATGP1032 |
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| Order / More Info | nanA, 1-297aa, E.coli, His tag, E.coli from ATGen |
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| Product Specific References | n/a |
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