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| Product Name | Tissue cDNA, First Strand, Human Adult Normal, Brain, Hippocampus, BioGenomics |
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| Description | Molecular Biology Grade. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 1 1 ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul tinal volume. RT Reaction stol2ped by heati.ng at 65 degree C for 10 minutes. The cDNA IS In 1 X RT buffer. (1 X RT Buffer: 50mM Tris-CI, pH 8:1. 75mM KCI, 3mM MgCI2, 10mM D I I). The estimated cDNA concentration is about 2.5ng/ul. 1 ul cDNA is sufficient for one PCR rea |
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| Size | 10 rxn |
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| Concentration | 2.5ng/ul |
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| Applications | Immediate PCR Amplification of known genes, Verification of genetic mutation, Comparison of a specific gene between different tissues, Analysis of mRNA alternative splicing, Gene cloning and target sequencing |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | MBS651929 |
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| Order / More Info | Tissue cDNA, First Strand, Human Adult Normal, Brain, Hippocampus, BioGenomics from MYBIOSOURCE INC. |
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| Product Specific References | n/a |
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