| 产品详情 |
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| Product Name | NFkB (p50) ELISA Kit |
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| Description | NFkB (P50) ELISA Kit - KAA064 |
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| Size | 1 kit |
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| Concentration | n/a |
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| Applications | ELISA. Rockland's NF-kB (p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate (see Figure 1 on page 4). NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p50) is detected by addition of a specific primary antibody directed against NF-kB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Rockland's NF-kB (p50) Transcription Factor Assay detects human NF-kB (p50). It will not cross-react with NF-kB (p65). |
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| Other Names | NF-kB Transcription Factor Kit, p50 kit, EIA kit, NF kappaB ELISA Assay |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | KAA064 |
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| Price | |
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| Order / More Info | NFkB (p50) ELISA Kit from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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