| 产品详情 |
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| Product Name | Recombinant Vaccinia virus DNA topoisomerase 1B (TOP1) |
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| Description | Purity > 90% as determined by SDS-PAGE. MW: 40.7kDa. Full Length: 1-314aa. N-terminal 6xHis-tagged. Vaccinia virus (strain Copenhagen) (VACV). Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. |
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| Size | 100ug |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | DNA topoisomerase I (Late protein H6) (TopIB) |
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| Gene, Accession, CAS # | TOP1, UniProt: P68697 |
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| Catalog # | RPC26203 |
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| Order / More Info | Recombinant Vaccinia virus DNA topoisomerase 1B (TOP1) from BIOMATIK |
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| Product Specific References | n/a |
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