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Product Name | Protease S. aureus |
Description | Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay. |
Size | 1 mg |
Concentration | 1 mg/mL |
Applications | Protease S. aureus has shown that one unit causes a change of 0.001 A280 nm per minute at 37° C at pH 7.8 using casein as a substrate. Specific conditions for reactivity should be optimized by end user. |
Other Names | Glutamyl endopeptidase,Endoproteinase Glu-C, Staphylococcal serine proteinase, V8 protease, V8 proteinase |
Gene, Accession, CAS # | sspA, NCBI: 1280, UniProt: P0C1U8 |
Catalog # | MB-114-0001 |
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Order / More Info | Protease S. aureus from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |
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