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| Product Name | Recombinant Heron hepatitis B virus Protein P (P), partial |
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| Description | Purity > 85% as determined by SDS-PAGE. MW: 28.7kDa. Partial: 376-565aa. N-terminal 10xHis-tagged and C-terminal Myc-tagged. Heron hepatitis B virus (HHBV). Multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3'- to 5'-endonucleasic mode. Neo-synthesized pregenomic RNA (pgRNA) are encapsidated together with the P protein, and reverse-transcribed inside the nucleocapsid. Initiation of reverse-transcription occurs first by binding the epsilon loop on the pgRNA genome, and is initiated by protein priming, thereby the 5'-end of (-)DNA is covalently linked to P protein. Partial (+)DNA is synthesized from the (-)DNA template and generates the relaxed circular DNA (RC-DNA) genome. After budding and infection, the RC-DNA migrates in the nucleus, and is converted into a plasmid-like covalently closed circular DNA (cccDNA). The activity of P protein does not seem to be necessary for cccDNA generation, and is presumably released from (+)DNA by host nuclear DNA repair machinery |
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| Size | 100ug |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | n/a |
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| Gene, Accession, CAS # | P, UniProt: P13846 |
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| Catalog # | RPC25943 |
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| Order / More Info | Recombinant Heron hepatitis B virus Protein P (P), partial from BIOMATIK |
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| Product Specific References | n/a |
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