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| Product Name | 25 Hydroxy Vitamin D BioAssay™ ELISA Kit (Human) |
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| Description | Sample Type: Serum, plasma and other biological fluids. Intended Use: Competitive ELISA, Coated with Antigen Sensitivity: <1.875ng/ml Range: 3.125-200ng/ml Specificity: Human Test Principle: This BioAssay™ ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 25 Hydroxy Vitamin D. During the reaction, 25 Hydroxy Vitamin D in the sample or standard competes with a fixed amount of 25 Hydroxy Vitamin D on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 25 Hydroxy Vitamin D. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of 25 Hydroxy Vitamin D in the |
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| Size | 48 tests, 96 tests |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 369682 |
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| Order / More Info | 25 Hydroxy Vitamin D BioAssay™ ELISA Kit (Human) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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