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Product Name | 25-Hydroxyvitamin D3 (HVD3) BioAssay™ ELISA Kit |
Description | The 25-Hydroxy Vitamin D3 (HVD3) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of HVD3 in serum, plasma and other biological fluids. Detection Range: 2.47-200ng/ml Sensitivity: <0.88ng/ml Precision: Intra-Assay CV: <10% Inter-Assay CV: <12% Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for HVD3 has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled HVD3 and unlabeled HVD3 (standards or samples) with the pre-coated antibody specific for HVD3. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of HVD3 in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration |
Size | 96 tests |
Concentration | n/a |
Applications | n/a |
Other Names | n/a |
Gene, Accession, CAS # | n/a |
Catalog # | 23031 |
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Order / More Info | 25-Hydroxyvitamin D3 (HVD3) BioAssay™ ELISA Kit from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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