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| Product Name | Biotin Quantitation BioAssay™ Kit (Colorimetric) |
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| Description | Biotin (Vitamin H) is an important biomolecule that has been widely used in Biotinylation reactions to label antibodies or other proteins of interest. Due to its low molecular weight, biotin usually does not cause any significant changes in protein conformation and biological activity. Biotinylated proteins/antibodies are widely isolated and assayed with streptavidin pull-down assay, affinity chromatography, ELISA and Western blotting etc. It is often desired to determine the degree of substitution of such biotinylated biomolecules. Intended Use: For use in measuring free biotin or the substitution degree of Biotinylation (biotin labels) of proteins and antibodies. Sensitivity: This kit can be used to estimate as low as 60pmol or 15ng of biotin in solution. Test Principle: The Biotin BioAssay™ Kit is based on the differential binding of Streptavidin to Biotin and a dye, 2-(4-hydroxyazobenzene) benzoic acid (HABA). When HABA is bound to Streptavidin, addition of free biotin or bio |
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| Size | 100 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 219467 |
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| Order / More Info | Biotin Quantitation BioAssay™ Kit (Colorimetric) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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