| 产品详情 |
| Edit |   |
|---|
| Product Name | Caspase-1/ICE Assay Kit (Colorimetric) |
|---|
| Description | Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity. Sample types: cell lysates, tissue homogenates |
|---|
| Size | n/a |
|---|
| Concentration | n/a |
|---|
| Applications | This assay provides for the detection of early/middle stages of apoptosis; differentiate apoptosis from necrosis. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity. |
|---|
| Other Names | n/a |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | LS-K5 |
|---|
| Price | |
|---|
| Order / More Info | Caspase-1/ICE Assay Kit (Colorimetric) from LIFESPAN BIOSCIENCES INC. |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|