| 产品详情 |
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| Product Name | Flex-T™ HLA Class I ELISA Control |
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| Description | The Flex-T™ system is designed to provide maximal flexibility in the study of antigen specific T cells. Flex-T™ Monomer UVX products are a biotinylated HLA class I α-chain, associated with the β2-microglobulin chain, and stabilized by a UV-labile peptide. The C-terminus of the α-chain is biotinylated by the enzyme BirA. In the absence of proper peptide binding, the HLA class I complex quickly disassociates. Thus, by using UV radiation, it is possible to exchange the labile peptide with another peptide of interest. An ELISA was designed to verify this exchange process. A 96-well plate coated with Streptavidin captures the biotinylated α-chain, followed by detection of the associated β2-microglobulin with HRP-conjugated anti-β2-microglobulin antibody. The Flex-T™ HLA Class I ELISA Control is a HLA class I α-chain associated with β2-microglobulin and stabilized with a high-affinity, non UV-labile peptide. This stable monomer serves as positive control for this ELISA. |
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| Size | 20 µg |
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| Concentration | n/a |
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| Applications | ELISA, Flow Cytometry (FC) |
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| Other Names | MHC tetramer, HLA tetramer, HLA monomer, MHC monomer, biotinylated HLA monomer |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 280301 |
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| Order / More Info | Flex-T™ HLA Class I ELISA Control from BIOLEGEND |
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| Product Specific References | n/a |
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