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| Product Name | Caspase-3 Substrate DEVD-AFC |
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| Description | Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. MW: 729. Purity >98% by HPLC |
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| Size | 1000 assays |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | Ac-DEVD-AFC, Caspase-3 Substrate Fluorogenic |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 1007-1000 |
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| Order / More Info | Caspase-3 Substrate DEVD-AFC from BIOVISION, INC. |
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| Product Specific References | n/a |
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