| Edit |   |
| Antigenic Specificity | Rad9 |
| Clone | polyclonal |
| Host Species | Rabbit |
| Reactive Species | yeast |
| Isotype | n/a |
| Format | immunogen affinity purified |
| Size | 0.1 mg |
| Concentration | 0.74 mg/ml |
| Applications | Western Blot, ELISA. This pan reactive polyclonal antibody was tested by immunoblotting and ELISA. Data from both immunoblotting and ELISA indicate the antibody is pan reactive with both the phosphorylated and non-phosphorylated forms of the peptide and protein. Immunoblotting detects yeast Rad9 protein. No reactivity is expected against human and mouse homologs. Reactivity - Rad9 from others sources is unknown. Although not tested, this antibody is likely functional by immunohistochemistry and immunoprecipitation.This product has been assayed against 0.1 ug of immunizing peptide in a standard capture ELISA using TMB TMBE-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 is suggested for this product. Reactivity was detected against both the phosphorylated and non-phosphorylated form of the immunizing peptide. This antibody appears - be pan reactive for both forms of the protein. Dilute the antibody 1:100 - 1:500 for immunoblotting. Researchers should determine optimal titers for other applications. |
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| Description | The Rad9 Antibody from Novus Biologicals is a rabbit polyclonal antibody to Rad9. This antibody reacts with yeast. The Rad9 Antibody has been validated for the following applications: Western Blot, ELISA. This affinity purified antibody is directed against an internal sequence of yeast Rad9 at the S1129 residue |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 1125-1139 of 1309 of yeast Rad9 protein conjugated to KLH. |
| Other Names | cell cycle checkpoint control protein RAD9A, DNA repair exonuclease rad9 homolog A, EC 3.1.11.2, hRAD9, RAD9, RAD9 (S. pombe) homolog, RAD9 homolog A (S. pombe) |
| Gene, Accession # | RAD9A, Gene ID: 5883, Accession: Q99638, SwissProt: Q99638 |
| Catalog # | NBP1-77978 |
| Price | |
| Order / More Info | Rad9 Antibody from NOVUS BIOLOGICALS, LLC |
| Product Specific References | n/a |