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Product Name | DNA Marker 1kb Ladder, 14 Fragments (250-10,000bp), BioGenomics™ |
Description | DNA ladder was prepared from six different plasmids containing pUC, lambda phage and yeast genome sequences. Each plasmid was individually digested by the appropriate restriction endonucleases. The digests were then mixed. The marker yields the following 14 discrete fragments (in base pairs): 10,000, 8000, 6000, 5000, 4000, 3500, 3000, 2500, 2000, 1500, 1000, 750, 500, 250. Band Concentration: The band at 6000, 3000bp serves as a reference. 1ug of DNA Ladder Mix contains 70ng (~14% of total) of the 6000, 3000bp fragment. 1ug of DNA Ladder Mix contains 60ng (~12% of total) of the 1000bp fragment. 6X Loading Dye Solution (L3350): Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60mM EDTA. Quality Control: Analysis of 0.5ug (1ul) of the DNA ladder on agarose gel by ethidium bromide staining generates 14 descrete bands pattern. Recommendations for Use: 1. Vortex gently just prior to use. 2. Prepare the DNA marker before loading by |
Size | 5x50ug |
Concentration | n/a |
Applications | n/a |
Other Names | Supplied with 6x Loading Dye Solution |
Gene, Accession, CAS # | n/a |
Catalog # | D3930-11 |
Price | |
Order / More Info | DNA Marker 1kb Ladder, 14 Fragments (250-10,000bp), BioGenomics™ from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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