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Product Name | DNA Marker Lambda EcoRI, 6 Fragments (3530-21226bp), BioGenomics™ |
Description | Lambda DNA was completely digested by EcoRI, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl, pH 7.6, 1mM EDTA. The marker yields the following 6 discrete fragments (in base pairs): 21226*, 7421, 5804, 5643, 4878, 3530*. *The cohesive ends of the 12 nt cos site of bacteriophage lambda from fragments of 21226 bp and 3530 bp may anneal and form an additional band at 24756 bp. These fragments may be separated by heating at 65°C for 5 minutes and then cooling on ice for 3 minutes. 6x Loading Dye Solution (L3350): Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60mM EDTA. Quantity of DNA in band: Fragment % ng DNA (bp) 21226 43.8 218.8 7421 15.3 76.5 5804 12.0 59.8 5643 11.6 58.2 4878 10.1 50.3 3530 7.3 36.4 Total 100 500 Note: 1.One vial is sufficient for ~100 applications. 2. Use 0.1ug of DNA marker (before dilution) per 1mm and an agarose gel lane. Recommended Use: Vortex gently prior to use. Prepare DNA M |
Size | 5x50ug |
Concentration | n/a |
Applications | n/a |
Other Names | Supplied with 6x Loading Dye Solution |
Gene, Accession, CAS # | n/a |
Catalog # | D3930-17 |
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Order / More Info | DNA Marker Lambda EcoRI, 6 Fragments (3530-21226bp), BioGenomics™ from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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