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Product Name | DNA Marker Lambda EcoRI, HindIII, 13 Fragments (125-21226bp), BioGenomics™ |
Description | Lambda DNA was completely digested by both EcoRI and HindIII, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 7.6), 1mM EDTA. The marker yields the following 13 discrete fragments (in base pairs): 21226*, 5148, 4973, 4268, 3530*, 2027, 1904, 1584, 1375, 947, 831, 564, 125. The cohesive ends of the 12 nt cos site of bacteriophage lambda from fragments of 21226 bp and 3530 bp (indicated*) may anneal and form an additional band at 24756 bp. These fragments may be separated by heating at 65°C for 5min and then cooling on ice for 3min. Prepare DNA Marker as follows: 1ul (0.5ug) DNA marker 1ul of 6X Loading Dye Solution 4ul ddH2O 1. Vortex gently prior to use 2. Heat 5 min. at 65°C, cool on ice 3 min. 3. Apply 6ul DNA marker per 5mm of agarose gel lane width. 4. Visualize bands with ethidium bromide staining. |
Size | 5x50ug |
Concentration | n/a |
Applications | n/a |
Other Names | Supplied with 6x Loading Dye Solution |
Gene, Accession, CAS # | n/a |
Catalog # | D3930-23 |
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Order / More Info | DNA Marker Lambda EcoRI, HindIII, 13 Fragments (125-21226bp), BioGenomics™ from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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