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Product Name | DNA Marker Lambda HindIII, 8 Fragments (125-23130bp), BioGenomics™ |
Description | Lambda DNA was completely digested by HindIII, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 7.6), 1mM EDTA. The marker yields the following 8 discrete fragments (in base pairs): 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125. *The cohesive ends of the 12nt cos site of bacteriophage lambda from fragments of 23130bp and 4361bp may anneal and form an additional band at 27491 bp. These fragments may be separated by heating at 65°C for 5min and then cooling on ice for 3min. 6x Loading Dye Solution (L3350): 0.2% bromophenol blue, 0.2% xylene cyanol, 60% glycerol, 60mM EDTA. Fragment (bp) % ng DNA/0.5ug 23130 47.7 238.4 9416 19.4 97.1 6557 13.5 67.6 4361 9.0 45.0 2322 4.8 23.9 2027 4.2 20.9 564 1.2 5.8 125 0.3 1.3 Note: One vial (0.05mg) is sufficient for ~100 applications. Use 0.1ug (0.2ul) of the DNA Marker (before dilution) per 1mm of agarose gel lane width. Recommended Use: Vortex gently prior to use. Prepare DNA Marker as follows: 1ul (0.5ug) D3930-20 1ul of |
Size | 5x50ug |
Concentration | n/a |
Applications | n/a |
Other Names | Supplied with 6x Loading Dye Solution |
Gene, Accession, CAS # | n/a |
Catalog # | D3930-20 |
Price | |
Order / More Info | DNA Marker Lambda HindIII, 8 Fragments (125-23130bp), BioGenomics™ from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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