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| Product Name | IL-8 (Interleukin 8) BioAssay™ ELISA Kit (Equine) |
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| Description | Sample Type: Serum, plasma, tissue homogenates and other biological fluids. Intended Use: Sandwich ELISA, Double Antibody Sensitivity: <9.375pg/ml Range: 15.625-1000pg/ml Specificity: Equine Test Principle: This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. IL-8 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-8 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-8 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-8 |
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| Size | 48 tests, 96 tests |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 370039 |
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| Order / More Info | IL-8 (Interleukin 8) BioAssay™ ELISA Kit (Equine) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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