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Product Name | IL-2 (Interleukin 2) BioAssay™ ELISA Kit (Hamster) |
Description | Sample Type: Serum, plasma, tissue homogenates and other biological fluids. Intended Use: Sandwich ELISA, Double Antibody Sensitivity: <7.5pg/ml Range: 12.5-800pg/ml Specificity: Hamster Test Principle: This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. IL-2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-2 can |
Size | 48 tests, 96 tests |
Concentration | n/a |
Applications | ELISA |
Other Names | n/a |
Gene, Accession, CAS # | n/a |
Catalog # | 370027 |
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Order / More Info | IL-2 (Interleukin 2) BioAssay™ ELISA Kit (Hamster) from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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