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| Product Name | OxiSelect Cellular UV-Induced DNA Damage Staining Kit (6-4PP) |
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| Description | Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing 6-4PP damage are probed with an anti-6-4PP antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope. Background: Absorption of ultraviolet (UV) light produces two predominant types of DNA damage, cyclobut |
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| Size | 32 assays, 96 assays, |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | MBS168553 |
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| Order / More Info | OxiSelect Cellular UV-Induced DNA Damage Staining Kit (6-4PP) from MYBIOSOURCE INC. |
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| Product Specific References | 1. Lippke JA, Gordon LK, Brash DE, Haseltine WA. (1981) Proc Natl Acad Sci U S A. 78:3388-3392.2. Mitchell DL, Nairn RS. (1989) Photochem Photobiol. 49:805-819.3. Ananthaswamy HN, Loughlin SM, Cox P, Evans RL, Ullrich SE, Kripke ML. (1997) Nat Med. 3:510-514.4. Soehnge H, Ouhtit A, Ananthaswamy ON. (1997) Front Biosci. 2:D538-D551.5. el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW, Vogelstein B. (1993) Cell. 75:817-825.6. Hermeking H, Lengauer C, Polyak K, He TC, Zhang L, Thiagalingam S, Kinzler KW, Vogelstein B. (1997) Mol Cell. 1:3-11.7. Hill LL, Ouhtit A, Loughlin SM, Kripke ML, Ananthaswamy HN, Owen-Schaub LB. (1999) Science. 285:898-900. |
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