| 产品详情 |
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| Product Name | OxiSelect Methylglyoxal (MG) Competitive ELISA Kit |
| Description | First, an MG conjugate is coated on the ELISA plate. The unknown MG protein samples or MG-BSA standards are then added to the MG conjugate preabsorbed plate. After a brief incubation, the anti-MG antibody is added, followed by an HRP conjugated secondary antibody. The content of MG protein adducts in unknown samples is determined by comparison with the predetermined MG-BSA standard curve. Background: The non-enzymatic reaction of reducing carbohydrates with lysine side chains and N-terminal amin |
| Size | 96 assays, 480 assays, |
| Concentration | n/a |
| Applications | n/a |
| Other Names | n/a |
| Gene, Accession, CAS # | n/a |
| Catalog # | MBS168996 |
| Price | |
| Order / More Info | OxiSelect Methylglyoxal (MG) Competitive ELISA Kit from MYBIOSOURCE INC. |
| Product Specific References | 1. Shamshi F. A, Partal A, Sady C, Glomb M. A, Nagaraj R. H (1998) J Biol Chem 273:6928-6936.2. Cai W, Gao Q. D, Zhu L, Peppa M, He C, Vlassara H. (2002), Mol Med. 8:337-46.3. Monnier, V., and Cerami, A. (1981) Science 211, 491-493.4. Ahmed M.U., Thorpe S.R., Baynes J.W (1986) J. Biol. Chem. 261, 4889-4894.5. Reddy S., Bichler J., Wells-Knecht K.J., Thorpe S.R., Baynes J.W (1995) Biochemistry 34, 10872-10878.6. Dunn, J. A., Patrick, J. S., Thorpe, S. R., and Baynes, J. W. (1989) Biochemistry 28, 9464-9467. Ahmed, M. U., Brinkmann Frye, E., Degenhardt, T. P., Thorpe, S. R., and Baynes, J. W. (1997) Biochem. J. 324, 565-570.8. Sell, D. R., and Monnier, V. M. (1989) J. Biol. Chem. 264, 21597-21602.9. Onorato, J., Jenkins, A., Thorpe, S., and Baynes, J. (2000) J. Biol. Chem. 275, 21177-21184.10. Boehm BO, Schilling S, Rosinger S, Lang GE, Lang GK, Kientsch-Engel P, Stahl P (2004) Diabetologia 47, 1376-1379.Morgan, P. E. et al. (2014). Perturbation of human coronary artery endothelial cell redox state and NADPH generation by methylglyoxal. PLoS One. 9:e86564. |
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