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| Product Name | OxiSelect Nepsilon-(carboxymethyl) lysine (CML) Competitive ELISA Kit |
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| Description | First, a CML conjugate is coated on the ELISA plate. The unknown CML protein samples or CML-BSA standards are then added to the CML conjugate preabsorbed plate. After a brief incubation, the anti-CML monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CML protein adducts in unknown samples is determined by comparison with the predetermined CML-BSA standard curve. Background: The non-enzymatic reaction of reducing carbohydrates with lysine side chains an |
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| Size | 32 assays, 96 assays, 480 assays, |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | MBS168563 |
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| Price | |
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| Order / More Info | OxiSelect Nepsilon-(carboxymethyl) lysine (CML) Competitive ELISA Kit from MYBIOSOURCE INC. |
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| Product Specific References | 1. Monnier, V., and Cerami, A. (1981) Science 211, 491-493.2. Ahmed M.U., Thorpe S.R., Baynes J.W (1986) J. Biol. Chem. 261, 4889-4894.3. Reddy S., Bichler J., Wells-Knecht K.J., Thorpe S.R., Baynes J.W (1995) Biochemistry 34, 10872-10878.4. Dunn, J. A., Patrick, J. S., Thorpe, S. R., and Baynes, J. W. (1989) Biochemistry 28, 9464-9468.5. Ahmed, M. U., Brinkmann Frye, E., Degenhardt, T. P., Thorpe, S. R., and Baynes, J. W. (1997) Biochem. J. 324, 565-570.6. Sell, D. R., and Monnier, V. M. (1989) J. Biol. Chem. 264, 21597-21602.7. Onorato, J., Jenkins, A., Thorpe, S., and Baynes, J. (2000) J. Biol. Chem. 275, 21177-21184.8. Boehm BO, Schilling S, Rosinger S, Lang GE, Lang GK, Kientsch-Engel P, Stahl P (2004) Diabetologia 47, 1376-1379.9. Koito W, Araki T, Horiuchi S, Nagai R (2004) J. Biochem. 136, 831-837.1. Niquet-Leridon, C. et al. (2015). The rehabilitation of raw and brown butters by the measurement of two of the major Maillard Products, Nepsilon-carboxymethyl-lysine and 5-hydroxymethylfurfural, with validated chromatographic methods. Food Chemistry. 177:361-368.2. Huang, T. C. et al. (2014). Increased renal semicarbazide-sensitive amine oxidase activity and methylglyoxal levels in aristolochic acid-induced nephrotoxicity. Life Sci. 114:4-11.3. Morgan, P. E. et al. (2014). Perturbation of human coronary artery endothelial cell redox state and NADPH generation by methylglyoxal. PLoS One. 9:e86564. |
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