| 产品详情 |
| Edit |   |
|---|
| Product Name | OxiSelect Protein Carbonyl Fluorometric Assay |
|---|
| Description | Protein carbonyls in protein samples (1-10 mg/mL) are first derivatized with the Protein Carbonyl Fluorophore. The Fluorophore binds to the protein carbonyl group in a 1:1 ratio. Proteins are then TCA precipitated and free Fluorophore is removed by washing the protein pellet with acetone. After dissolving the protein pellet in GuHCl, the absorbance of protein-fluorophore product is measured fluorometrically with a 485/538 nm filter set, and the protein carbonyl is subsequently calculated. Backgr |
|---|
| Size | 20 assays, 100 assays, |
|---|
| Concentration | n/a |
|---|
| Applications | n/a |
|---|
| Other Names | n/a |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | MBS168032 |
|---|
| Price | |
|---|
| Order / More Info | OxiSelect Protein Carbonyl Fluorometric Assay from MYBIOSOURCE INC. |
|---|
| Product Specific References | 1. Buss, H, Chan, TP, Sluis, KB, Domigan, NM, and Winterbourn, CC.(1985) Free Radic Biol Med. 23: 361-6.2. Cadenas, E., Boveris, A., Ragan, CI., and Stoppani, AO. (1977) Archives of Biochemistry & Biophysics 180: 248257.3. Hammer, M., Schweitzer, D., Richter, S., Konigsdorffer, E. (2005) Physiol. Meas. 26: N9-N12.4. Reznick, AZ., and Packer, L. (1994) Methods Enzymol. 233: 263-357.5. Talent, JM., Kong, Y., and Gracy, RW. (1998) Anal. Biochem. 263: 3138.6. Wakeyama, H., Takeshige, K., Takayanagi, R., and Minakami, S. (1982) Biochem J. 205: 593601.Zabala, V. et al. (2015). Potential contributions of the tobacco nicotine-derived nitrosamine ketone (NNK) in the pathogenesis of steatohepatitis in a chronic plus binge rat model of alcoholic liver disease. Alcohol Alcohol. doi: http://dx.doi.org/10.1093/alcalc/agu083. |
|---|
| 产品资料 |
|
|