| 产品详情 |
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| Product Name | Bpu1102I (EspI) |
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| Description | 5'-G C^T N A G C-3' 3'-C G A N T^C G-5' Source: Bacillus pumilus RFL1102 Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. Concentration: 10 units/ul R1625 Restriction Enzyme Buffer A, 10X,: 33mM Tris-acetate, pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Double Digests: R1625 Restriction Enzyme Buffer A, 10X (Catalog No: R1625) is provided to simplify buffer selection for double digests. Y+/Tangle Buffer is supplied as a liquid in 33mM Tris-acetate, pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Incubate at 37°C. Dilution/Storage Buffer:: 10mM Tris-HCl, pH 7.4 at 25°C, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Quality Control: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Bpu1102I. Ligation/Recutting Assay: After 50-fold ove |
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| Size | 200U, 1000U |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | B2650 |
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| Order / More Info | Bpu1102I (EspI) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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