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| Antigenic Specificity | Ki-67/MKI67 |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human, mouse, rat, porcine |
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| Isotype | n/a |
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| Format | Alexa Fluor 488 conjugate |
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| Size | 0.1 ml |
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| Concentration | n/a |
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| Applications | Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. This Ki67 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections and Immunocytochemistry/Immunofluorescence. There have been mixed results in Western blot; however, NB500-170 has been used successfully Western Blog reported by a customer review. *Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval. This is probably the most critical step. NOTE: We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein (immunofluorescence may give problems as the protein is nuclear). |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The Ki-67/MKI67 Antibody [Alexa Fluor® 488] from Novus Biologicals is a rabbit polyclonal antibody to Ki-67/MKI67. This antibody reacts with human, mouse, rat, porcine. The Ki-67/MKI67 Antibody [Alexa Fluor® 488] has been validated for the following applications: Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. |
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| Immunogen | A synthetic peptide made to an internal region of human Ki67 (within residues 1550-1700) [Swiss-Prot# P46013] |
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| Other Names | antigen identified by monoclonal Ki-67, antigen KI-67, Ki67, Ki-67, KIA, Marker Of Proliferation Ki-67, MIB-1, PPP1R105, proliferation-related Ki-67 antigen, Protein Phosphatase 1, Regulatory Subunit 105 |
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| Gene, Accession # | MKI67, Gene ID: 4288, SwissProt: P46013 |
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| Catalog # | NB500-170AF488 |
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| Price | |
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| Order / More Info | Ki-67/MKI67 Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | PubMed: 26402156 |
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