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Product Name | Exonuclease III |
Description | Exonuclease III is a 3'-5' exonuclease releasing 5' mononucleotides from dsDNA and exhibits AP-specific endonuclease, 3' phosphatase and RNAse H activities. Source: E.coli cells carrying a cloned E.coli xth gene. Storage Buffer: Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 50mM KCl, 1mM DTT, 50% glycerol. Reaction Buffer (10X) E9008-05: Supplied as a liquid in 660mM Tris-HCl, pH 8.0, 6.6mM MgCl2 Unit Definition: One unit of Exonuclease III catalyzes the release of 1nmol of acid soluble reaction products from E. coli [3H]-DNA in 30 min at 37°C. Applications: Suitable for use in construction of nested unidirectional deletions of DNA fragments, generation of single-stranded template for DNA dideoxy sequencing, site-directed mutagenesis, cloning of PCR products and preparation of strand-specific probes. |
Size | 4000U |
Concentration | n/a |
Applications | n/a |
Other Names | Supplied with Reaction Buffer |
Gene, Accession, CAS # | n/a |
Catalog # | E9008 |
Price | |
Order / More Info | Exonuclease III from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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