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| Product Name | Recombinant Zaire ebolavirus RNA-directed RNA polymerase L (L), partial |
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| Description | Purity > 85% as determined by SDS-PAGE. MW: 27.9kDa. Partial: 625-809aa. N-terminal 10xHis-tagged and C-terminal Myc-tagged. Zaire ebolavirus (strain Kikwit-95) (ZEBOV). RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene. |
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| Size | 100ug |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Large structural protein |
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| Gene, Accession, CAS # | L, UniProt: Q6V1Q2 |
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| Catalog # | RPC23249 |
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| Order / More Info | Recombinant Zaire ebolavirus RNA-directed RNA polymerase L (L), partial from BIOMATIK |
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| Product Specific References | n/a |
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