| 产品详情 |
| Edit |   |
|---|
| Product Name | EP4 Receptor (rat) Activation Assay Kit (cAMP) |
|---|
| Description | The EP4 receptor is one of four GPCRs that mediate the actions of prostaglandin E2 (PGE2). The diverse effects of PGE2 acting via EP4 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Cayman's EP4 Receptor (rat) Activation Assay Kit (cAMP) consists of a 96-well plate coated with a transfection complex containing a DNA construct for rat EP4 Receptor (EP4 Reverse Transfection Strip Plate). Cells grown on the transfection complex will express EP4 at the cell surface. Binding of agonists to EP4 stimulates cAMP generation and increases intracellular cAMP levels, which can be measured by a competitive EIA using the Mouse Anti-Rabbit IgG Coated Plate and the reagents included in the kit. An EP4 agonist, PGE2, is included in the kit as a positive control. |
|---|
| Size | 1 ea |
|---|
| Concentration | n/a |
|---|
| Applications | Reverse Transfection Reporter Assay |
|---|
| Other Names | cell-based assays cells CBAs kits measures measurements prostaglandins E2 PGE2 prostanoids G protein-coupled receptors proteins GPCRs EP1 EP2 EP3 signals transductions mechanisms tissues localizations regulation expression intestines lungs kidneys thymus uterus brains cyclics AMP adenosines monophosphates pathways phosphatidylinositols 3-kinases G1 smooth muscles relaxation gastric secretions intestinal epithelial transportation adrenal aldosterone uterine immunity inflammation human colon cancers colorectal carcinogenesis in vivo neuroprotective excitotoxic injury injuries agonists antagonists therapeutics diseases surface transfections and expression protocols transient technology technologies University of Michigan UM U of M Originus, Inc. USPT United States patents 6897067 6902933 7056741 DNA deoxyribonucleic recombinant proteins cells receptor-mediated endocytosis solution-phase efficiency multiple plasmids constructs intracellular reverse reporter assay |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | 600410-1EA |
|---|
| Price | |
|---|
| Order / More Info | EP4 Receptor (rat) Activation Assay Kit (cAMP) from CAYMAN CHEMICAL COMPANY |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|