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Product Name | DAF-2 diacetate |
Description | Purity >95%. DAF-2 diacetate is a sensitive fluorescent indicator for the detection and bioimaging of nitric oxide (NO). It is a cell-permeable derivative of DAF-2. Upon entry into the cell, DAF-2 diacetate is transformed into the less cell-permeable DAF-2 by cellular esterases thus preventing loss of signal due to diffusion of the molecule from the cell. In the presence of oxygen, DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nm, respectively. At neutral pH the detection limit for NO is 2-5 nM. DAF-2 diacetate can be utilized in cells which produce small amounts of NO, such as endothelial cells, as well as in cells which generate large amount of NO, such as macrophages. |
Size | n/a |
Concentration | n/a |
Applications | n/a |
Other Names | 2-(3,6-diacetyloxy-4,5-diamino-9H-xanthen-9-yl)-benzoic acid, 4,5-Diaminofluorescein diacetate |
Gene, Accession, CAS # | CAS: 205391-02-2 |
Catalog # | LS-H7344 |
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Order / More Info | DAF-2 diacetate from LIFESPAN BIOSCIENCES INC. |
Product Specific References | n/a |
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