| 产品详情 |
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| Product Name | DAF-2 diacetate |
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| Description | DAF-2 diacetate is a sensitive fluorescent indicator for the detection and bioimaging of NO.{6360,6632} It is a cell-permeable derivative of DAF-2. Upon entry into the cell, DAF-2 diacetate is transformed into the less cell-permeable DAF-2 by cellular esterases thus preventing loss of signal due to diffusion of the molecule from the cell. In the presence of oxygen, DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nm, respectively.{6632} At neutral pH the detection limit for NO is 2-5 nM. DAF-2 diacetate can be utilized in cells which produce small amounts of NO, such as endothelial cells, as well as in cells which generate large amount of NO, such as macrophages.{6360,6632} |
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| Size | 250 µg |
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| Concentration | n/a |
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| Applications | Fluorescence |
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| Other Names | 4,5-Diaminofluorescein diacetate |
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| Gene, Accession, CAS # | CAS: 205391-02-2 |
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| Catalog # | 85165-250 |
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| Order / More Info | DAF-2 diacetate from CAYMAN CHEMICAL COMPANY |
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| Product Specific References | n/a |
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