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Product Name | JMJD2D, Chemiluminescent, BioAssay™ Kit (Jumonji Domain Containing 2D, KDM4D) |
Description | JMJD2D is a JmjC-domain protein that exhibits demethylation activity toward H3-K9Me3 and H3-K9Me2. Intended Use: The JMJD2D Chemiluminescent BioAssay™ Kit is designed to measure activity of JMJD2D for screening and profiling applications. Test Principle: The key to the JMJD2D Chemiluminescent BioAssay™ Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2D enzyme is incubated with a sample containing assay buffer for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt Applications: Great for studying enzyme kinetics, screening small molecular inhibito |
Size | 96 tests |
Concentration | n/a |
Applications | n/a |
Other Names | n/a |
Gene, Accession, CAS # | n/a |
Catalog # | 298418 |
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Order / More Info | JMJD2D, Chemiluminescent, BioAssay™ Kit (Jumonji Domain Containing 2D, KDM4D) from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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