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| Product Name | Lep (Leptin) BioAssay™ ELISA Kit (Bovine) |
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| Description | Sample Type: Serum, plasma, tissue homogenates and other biological fluids. Intended Use: For quantitative detection of Lep in serum, plasma, tissue homogenates and other biological fluids. Sensitivity: <0.188ng/ml Precision: Intra-Assay CV: <8% Inter-Assay CV: <10% Range: 0.313-20ng/ml Specificity: Bovine Test Principle: This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Lep antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Lep antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Lep amount of |
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| Size | 48 tests, 96 tests |
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| Concentration | n/a |
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| Applications | ELISA |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 396439 |
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| Order / More Info | Lep (Leptin) BioAssay™ ELISA Kit (Bovine) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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