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| Product Name | Recombinant Human AP-2 complex subunit mu (AP2M1) |
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| Description | Purity > 90% as determined by SDS-PAGE. MW: 65.7kDa. Full Length: 1-435aa. N-terminal 6xHis-SUMO-tagged. Homo sapiens (Human). Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different mbrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to mbrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of mbranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the mbrane proteins involved in receptor-mediated endocytosis. AP-2 ses to play a role in the recycling of synaptic vesicle mbranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmbrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmbrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in mbrane-associated AP-2. The mbrane-specific phosphorylation event appears to involve assbled clathrin which activates the AP-2 mu kinase AAK1. Plays a role in endocytosis of frizzled family mbers upon Wnt signaling. |
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| Size | 100ug |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | AP-2 mu chain; Adaptin-mu2; Adaptor protein complex AP-2 subunit mu; Adaptor-related protein complex 2 subunit mu; Clathrin assembly protein complex 2 mu medium chain; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; HA2 50KDA subunit; Plasma membrane adaptor AP-2 50KDA protein |
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| Gene, Accession, CAS # | AP2M1, UniProt: Q96CW1 |
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| Catalog # | RPC20910 |
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| Order / More Info | Recombinant Human AP-2 complex subunit mu (AP2M1) from BIOMATIK |
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| Product Specific References | n/a |
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