| 产品详情 |
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| Product Name | Sperm Antibody ELISA Kit |
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| Description | An enzyme immunoassay for the quantitative determination of antibodies directed against spermatozoa antigens in serum. The Sperm Antibody ELISA (Enzyme Linked ImmunoSorbent Assay) is a solid-phase sandwich enzyme-immunoassay for the quantitative determination of anti-spermatozoa antibodies in human serum. The ELISA-plate is coated with a mix of spermatozoa proteins which are recognized by anti-spermatozoa antibodies. The samples and standards are pipetted into the wells and then incubated. During this incubation anti-spermatozoa antibodies bind to the spermatozoa proteins and are thus immobilised on the plate. After washing the enzyme conjugate, consisting of anti-human globulin antibodies covalently coupled to horseradish peroxidase, is added. After removal of the unbound conjugate by washing the horseradish peroxidase oxidizes the then added substrate TMB (3,3',5,5'-tetramethylbenzidine) yielding a color reaction which is stopped with 0.25 M sulphuric acid (H2SO4). The extinction is measured at a wavelength of 450 nm with a microplate reader. The use of a reference measurement with a wavelength ≥550 nm is recommended. |
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| Size | 96 wells |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | EIA, enzyme immunoassay, assay |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | EIA1826 |
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| Order / More Info | Sperm Antibody ELISA Kit from DRG INTERNATIONAL INC. |
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| Product Specific References | n/a |
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