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Product Name | PRMT3, Chemiluminescent, BioAssay™ Kit (Protein Arginine Methyltransferase 3, HRMT1L3) |
Description | PRMT3 is a histone methyltransferase that exhibits methylation activity toward H4-R3. Intended Use: The PRMT3 Direct Activity Chemiluminescent BioAssay™ kit is designed to measure PRMT3 activity for screening and profiling applications. Test Principle: The key to the PRMT3 Direct Activity BioAssay™ Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt Applications: Great for studying enzyme kinetics, screening small molecula |
Size | 96 tests |
Concentration | n/a |
Applications | n/a |
Other Names | n/a |
Gene, Accession, CAS # | n/a |
Catalog # | 298450 |
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Order / More Info | PRMT3, Chemiluminescent, BioAssay™ Kit (Protein Arginine Methyltransferase 3, HRMT1L3) from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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