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| Product Name | LDH-Cytotoxicity Fluorometric Assay Kit |
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| Description | Lactate Dehydrogenase (LDH, EC 1.1.1.27) is a stable enzyme and is present in all cell types. Cell damage leads to release of LDH enzyme into the media and its activity is widely used as a marker for cytotoxicity. It reversibly converts lactate into pyruvate, with the concomitant interconversion of NADH and NAD+. LDH-Cytotoxicity Fluorometric Assay Kit provides a sensitive, quick, and easy way for detection of LDH released from damaged cells. In this assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm). The amount of fluorescence is directly proportional to the number of damaged cells. The assay is adaptable to high-throughput format and can be completed in less than 20 min. Sensitivity: ~ 100 cells. |
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| Size | 500 assays |
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| Concentration | n/a |
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| Applications | Measurement of released LDH in culture medium. Evaluation of cytotoxicity effect of test compounds. |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | Kit-2194 |
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| Order / More Info | LDH-Cytotoxicity Fluorometric Assay Kit from CREATIVE BIOMART |
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| Product Specific References | n/a |
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