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| Product Name | HAHA (Human Anti-Human Antibody) ELISA Assay Kit Assay Kit |
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| Description | The Eagle Biosciences HAHA ELISA Assay Kit is designed, developed and produced for the quantitative measurement of HAHA in serum and plasma samples. The assay utilizes the two-site ``sandwich'' technique with two selected antibodies that bind to HAHA. Assay standards, controls and patient samples are directly added to wells of a microplate that is coated with highly purified human IgG. After the first incubation period, the HAHA binds to the human IgG on the wall of microtiter well and unbound proteins in each microtiter well are washed away. Then a horseradish peroxidase (HRP)-labeled human antibody is added to each microtiter well and a ``sandwich'' of ``well-coated human IgG - HAHA - HRP-conjugated human antibody'' is formed. The unbound HRP-conjugated human antibody is removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to HAHA on the wall of the microtiter well is directly proportional to the amount of HAHA in the sample. A standard curve is generated by plotting the absorbance versus the respective HAHA concentration for each standard on point-to-point or 4 parameter curve fit. The concentration of HAHA in test samples is determined directly from this standard curve. Sample Types: serum, plasma. Range: 0 - 27 ug/mL. Sensitivity: 0.1 ug/mL. Time: 2 hours • Clinically, humanized monoclonal antibodies (IgG) and their fragments are used in vivo diagnosis procedure (radionuclides) and treatment for patients with various diseases. In patients, even a single dose injection of a humanized monoclonal antibody or its fragment may induce immune response directed against this foreign protein (immunogen). Also, people with autoimmune diseases, such as rheumatoid arthritis, lupus, etc. produce autoantibody against human IgG. In the circulation, the presence of human antibody against human IgG would bind to the injected humanized antibody therapeutics or diagnosis and, therefore, diminish the efficacy of either in-vivo diagnosis or treatment. Especially, the HAHA would increase the risk of anaphylactic complications to subsequent administration of the humanized monoclonal antibody-based therapy. The presence of HAHA in patient serum or plasma specimens may cause both false positive and false negative immunoassay test results depending on assay principles and monoclonal antibodies used in the assay system. This Eagle Biosciences HAHA ELISA Assay Kit is a ready-to-use test kit with a breakable well microtiter plate and simple test procedures. It also provides a wide measurement range without high dose ``hook'' effect. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | HAHA, Human Anti-Human Antibody |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | HAH31-K01 |
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| Order / More Info | HAHA (Human Anti-Human Antibody) ELISA Assay Kit Assay Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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