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| Product Name | Human GIP(Active) ELISA |
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| Description | This Human GIP (Active) ELISA Assay kit for determination of human GIP (1-42) active form is based on a sandwich enzyme immunoassay. To the wells of plate coated with highly purified mouse monoclonal antibody against human GIP (1-42) active form, standards or samples are added for the 1st step immunoreaction. After the 1st step incubation and plate washing, HRP labeled antibody solution against human GIP (1-42) is added as the 2nd step to form antibody - Antigens - labeled antibody complex on the surface of the wells. After the 2nd step incubation and rinsing out excess labeled antibody, Finally, HRP enzyme activity is determined by 3,3',5,5'-Tetramethylbenzidine (TMB) and the concentration of human GIP (1-42) active form is calculated. Sample Types: plasma, cell culture supernatant. Range: 3.9 - 250 pg/mL. Time: 3.5 hours • The incretin hormones, glucose-dependent insulinotropic polypeptide (GIP) and glucagons-like peptide-1 (GLP-1), are a group of gastrointestinal hormones that cause an increase in the amount of insulin released from the beta cells of the islets of Langerhans after ingestion of food. The intestinal peptide GIP was first isolated from porcine upper small intestine1). The sequences of porcine2) 3), bovine4) and human GIP5) have been determined, each has 42 amino acids, and the sequences is highly conserved. The porcine and bovine peptides differ from the human at two and three site, respectively. Takeda et al. have isolated a human cDNA encoding the GIP precursor and confirming that GIP belongs to the vasoactive intestinal peptide (VIP)/Glucagon/secretin family6). GIP is a gastrointestinal peptide hormone that is released from duodenal Endocrinology K cells after absorption of glucose or fat7). GIP is a potent releaser of insulin in experimental animals8) and in man 9,10) provided that the blood glucose is above basal level. Plasma level of GIP is elevated after an oral glucose load or a meal in normal man. This increase after a meal is below normal in newly diagnosed insulin-dependent diabetics11). It is now being recognized that GIP receptor is also expressed in organs and cells such as duodenum, small intestine, pancreatic alpha-cell, adipocyte and osteoblast. These results demonstrate GIP may have a lot of physiological effect in addition to their glucoregulatory effects12,13,14,15). GIP is rapidly inactivated by the enzyme dipeptidyl peptidase- 4 (DPP- 4) to GIP (3-42) with a blood half-life of only several minutes. DPP- 4 inhibitor can prolong the half-life of GIP, that expecting treatment of incretin effect. This Eagle Biosciences Human GIP (Active) ELISA Assay kit has high specificity to human GIP (1-42) active form and shows no cross-reactivity to human GIP (3-42) inactive form. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Human GIP (Active) ELISA, Sceti, YK250 |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | YK250 |
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| Order / More Info | Human GIP(Active) ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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