| 产品详情 |
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| Antigenic Specificity | FLAG™ conjugated proteins |
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| Clone | 29E4.G7 |
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| Host Species | Mouse |
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| Reactive Species | n/a |
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| Isotype | IgG2a kappa |
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| Format | Protein A purified |
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| Size | 25 µL |
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| Concentration | 1.0 mg/mL |
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| Applications | ELISA, Flow Cytometry, Immunohistochemistry, Western Blot. This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This product is an IgG fraction antibody purified from ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. The purified antibody is directed against the FLAG™ motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins. This monoclonal anti-FLAG™ tag antibody detects over-expressed proteins containing the FLAG™ epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. |
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| Immunogen | This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling. |
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| Other Names | mouse anti-FLAG™ tag, Enterokinase Cleavage Site (ECS), mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys |
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| Gene, Accession # | n/a |
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| Catalog # | 200-301-383S |
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| Price | |
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| Order / More Info | FLAG™ conjugated proteins Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Huang, Y., Chuang, A., Hao, H., Talbot, C., Sen, T., Trink, B., ... & Ratovitski, E. (2011). Phospho-?Np63? is a key regulator of the cisplatin-induced microRNAome in cancer cells. Cell Death & Differentiation, 18(7), 1220-1230. |
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