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| Product Name | Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate |
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| Description | FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate is an extremely sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). FemtoMax™ is designed for both western blotting and enzyme immunoassay (EIA) use. FemtoMax™ easily allows for the detection of femtogram (10-15) amounts of antigen using photographic film or other imaging methods, including highly sensitive CCD cameras. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of detection reagents and re-probed. Use the same blotting conditions for FemtoMax™ as you would when using Amersham ECL Plus™ Substrate or Pierce SuperSignal® West Femto Substrate. |
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| Size | 110 mL |
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| Concentration | n/a |
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| Applications | ELISA, Western Blot. Prepare FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate for use in microwell or membrane applications by mixing 1 mL of Luminol Reagent (Reagent A) with 1 mL of Reaction Buffer (Reagent B). Mix well. Protect from light. Larger or smaller volumes of the substrate can be prepared by mixing components at the same 1:1 ratio. FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate is a highly sensitive detection reagent. Always carefully optimize all components of individual assays (antigens, antibodies, conjugates...) to minimize background reactivity associated with non-specific binding. |
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| Other Names | Peroxidase Substrate, ECL HRP Substrate, Chemiluminescent Femtomax™ Super Sensitive horseradish peroxidase (HRP) Substrate For Microwell And/Or Membrane (2 Component System) |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | FEMTOMAX-110 |
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| Order / More Info | Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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