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| Antigenic Specificity | cadherin, DE- (extracellular domain) |
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| Clone | DCAD2 |
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| Host Species | Rat |
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| Reactive Species | Drosophila |
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| Isotype | IgG2a, kappa light chain |
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| Format | HYBRIDOMA |
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| Size | 1 vial cells |
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| Concentration | n/a |
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| Applications | Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Epitope: Between cadherin repeats 0 and 1. Suitable for use in immunostaining and immunoprecipitation. In western blot analysis, DCAD1, another rat monoclonal antibody to DE-cadherin, is more sensitive than DCAD2, although DCAD1 is not appropriate for staining. About requests of DCAD1, please contact Tadashi Uemura (tuemura@lif.kyoto-u.ac.jp). Conditions for immunostaining are described in our papers (Developmental Biology; Genes & Development 10, 659- 671); in addition please note the following comments. 1/ Fixatives (3.7% formaldehyde in PB or 4% paraformaldehyde in CMF & GBS containing 1mM CaCl2). Either fixation protocol works to stain embryos and discs. To stain cultured cells, only the 4% paraformaldehyde condition worked in our hands. We do not prefer any fix solutions including EDTA or EGTA, because cadherin is easily degraded under Ca++- free conditions. 2/ We suspect that the epitope may be sensitive to long exposure to alcohol. So, embryos stored in alcohol may not be stained. 3/ Photos should be taken under confocal microscope. 4/ HRP/DAB reaction has never given clear-cut signals for DE-cadherin at cell-cell contact sites. For experiments involving live cells and tissue, DCAD2 supernatant is available without the antimicrobial ProClin. Contact us at 319-335-3826 or dshb@uiowa.edu for special ordering instructions. |
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| Immunogen | Proteins co-immunoprecipitated with an anti-alpha-catenin mAb, DCAT-1: Full length protein |
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| Other Names | CG3722, E-cadh |
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| Gene, Accession # | shg, Gene ID: 37386, UniProt: Q24298 |
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| Catalog # | DCAD2 |
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| Price | |
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| Order / More Info | cadherin, DE- (extracellular domain) Antibody from DEVELOPMENTAL STUDIES HYBRIDOMA BANK |
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| Product Specific References | PubMed: 7958432, 25793441, 20495554, 20434988, 20410139, 20403348, 21145886, 21187384, 22921201, 8598294, 16224820, 10588889, 10079365, 19682089, 21041636, 12551956, 22825871, 16882972, 27442438, 27685172, 27696669, 7958432, 10588889, 10409711, 19682089, 7958432, 9247265, 10079365, 12551956 |
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