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| Antigenic Specificity | cadherin, DN- (extracellular domain) |
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| Clone | DN-Ex #8 |
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| Host Species | Rat |
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| Reactive Species | Drosophila |
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| Isotype | IgG2a |
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| Format | supernatant |
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| Size | 1.0 ml |
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| Concentration | n/a |
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| Applications | Immunofluorescence, Immunohistochemistry, Western Blot |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Epitope: extracellular region including CR2-CR8. Suitable to use in immunostaining and western blots. 1/ Staining of embryos and discs; both fixative 1 (3.7% formaldehyde in PBS) and fixative 2 (4% paraformaldehyde in CMF & GBS containing 1mM CaCl2 (see details in Dev. Biol. 165, 716-726)) work. 2/ Staining of S2 cells; fixative 1 doesn't work, use fixative 2. 3/ Staining of pupal or adult heads for light microscope; find details in our MCN paper and references therein such as Uchida et al. (4% paraformaldehyde-based fixative on ice for 1-2 hr) and Hoshino et al. (PLP-based fixative on ice for 1-2 hr). For better fixation, we cut pupal or adult heads into halves. 4? ImmunoEM of adult heads; find details in our MCN paper. We do not add EDTA or EGTA in any fixatives, because cadherins are easily degraded under Ca++- free conditions. Marker of neuropil |
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| Immunogen | Recombinant fusion protein containing the extracellular region of N-cadherin from cadherin repeats 2 to 8: Partial protein |
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| Other Names | CG7100, N-cadh |
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| Gene, Accession # | CadN, Gene ID: 35070, UniProt: O15943 |
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| Catalog # | DN-Ex #8 |
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| Price | |
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| Order / More Info | cadherin, DN- (extracellular domain) Antibody from DEVELOPMENTAL STUDIES HYBRIDOMA BANK |
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| Product Specific References | PubMed: 9247265, 9247265, 11906210, 21985007, 9247265, 20434990, 19711412, 23880429, 25779704, 26986723, 23240029, 21246549, 21985007, 20187144, 17029252, 18445681, 21524279, 22595515, 9247265 |
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