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| Product Name | Acid Sphingomyelinase (SMase) Activity Kit (Colorimetric) |
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| Description | Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. The Acid Sphingomyleinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples. Sample types: adherent cells, animal tissues, brain, heart, kidney, serum, suspension cells |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | Measurement of ASMase activity in various tissues/cell extractsScreening of ASMase inhibitors |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | LS-K600 |
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| Order / More Info | Acid Sphingomyelinase (SMase) Activity Kit (Colorimetric) from LIFESPAN BIOSCIENCES INC. |
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| Product Specific References | n/a |
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