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| Product Name | Normal Rat Brain Whole Cell Lysate |
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| Description | Ready-to-use whole cell lysates produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins. |
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| Size | 500 µg |
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| Concentration | 1.0 mg/mL |
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| Applications | WB. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 µl depending on the size format of your gel. |
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| Other Names | Normal Rat Brain Whole Cell Lysate, Rat Brain WCL, Rat Brain Lysate |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | W12-000-T077 |
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| Price | |
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| Order / More Info | Normal Rat Brain Whole Cell Lysate from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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