PRODUCT NUMBER:

Species Reactivity: Human Liver

Host: Rabbit

Immunogen Source: Human Liver

Form: Ig Fraction (Standard Antisera) obtained by ammonium sulfate precipitation.

Applications: ELISA, WB (not suitable for IEP)

Buffer: phosphate buffered saline with 0.05% NaN3 as a preservative

Storage: +2 to 8°C

Protein Concentration: 20.6 mg/mL

Working Dilution: >1:16000 by indirect ELISA.

Western Blot: At a dilution of 1:4000 the antibody reacts with 0.075, 0.15, and 0.3 ug human liver cathepsin D and does not react with cathepsins B, H or L.

The Cathepsin D immunogen used to prepare this antibody was purified in the Athens Research & Technology laboratory. It is a native protein, whole molecule, isolated from human liver.

PLEASE NOTE: This product is a laboratory reagent and is not to be administered to humans or used for any drug purpose. For research use only.

References:
Wӓster P, Rosdahl I, Ӧllinger K. Cell fate regulated by nuclear factor-κB- and activator protein-1-dependent signalling in human melanocytes exposed to ultraviolet A and ultraviolet B. Br J Dermatol. 2014 Dec;171(6):1336-46. doi: 10.1111/bjd.13278. Epub 2014 Nov 14.

Wäster P, Eriksson I, Vainikka L, Rosdahl I, Öllinger K. Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation. Sci Rep. 2016 Jun 13;6:27890. doi: 10.1038/srep27890.