CD20 is present on all normal B cells in peripheral blood, lymph nodes, spleen, tonsil and bone marrow. The antigen is also present on most B cell lines and on non-T ALL and CLL cells.
| Target |
CD20 |
| Reactivity |
Human |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Tested Applications |
FCM |
| Recommended dilutions |
FCM: 20 µl/1 million cells. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Normal human lymphocytes from lymph node. |
| Purification |
Affinity Chromatography |
| Form |
Liquid |
| Isotype |
IgG2a |
| Conjugation |
APC |
| Specificity |
CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82. |
| Storage |
Aliquot and store in the dark at 2°C to 8°C. Avoid exposure to light. |
| Molecular Weight |
37/35 kDa |
| Swiss Prot |
P11836
|
| GeneID |
931
|
| Buffer |
The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 3-5 working days. |
| Note |
This product is for research use only. |